Volume 1, Issue 1, June 2013, Page: 1-7
Antidiabetic Potential of Turmeric with/without Fermented Milk Enriched with Probiotics in Diabetic Rats
Badkook Maha, Department of Food and Nutrition, King AbdulAziz University, Jeddah, Saudi Arabia
Received: Apr. 26, 2013;       Published: May 30, 2013
DOI: 10.11648/j.ajbls.20130101.11      View  3196      Downloads  189
Purpose: To investigate the effect of turmeric supplement (T), fermented milk with Probiotic strain Bifidobacterium lactis DN-173 010 (P), a combination of (T+P) on glycemia, lipidemia, and oxidative status in streptozotocin–induced diabetic rats.Materials and methods: Thirty male albino diabetic rats were fed a normal diet and divided to groups (n=6/group) according to the following treatments for 45 days: Turmeric (T) (225mg/kg/d), fermented milk Probiotics (P) (0.5ml/kg/d), Turmeric-Probiotics combination (T+ P) (225mg/kg/d + 0.5ml/kg/d), Oral hypoglycemic agent (OHA) (0.5 ml of glibenclamide; 450 µg/kg/day), and non-treated (DM). Blood glucose and glycayted hemoglobin were determined after diabetes induction and at the end of experiment. Serum insulin, total cholesterol (TC), LDL-C, HDL-C, TG, malondialdehyde (MDA), and total antioxidant capacity (TOAC) biomarker of oxidative stress were determined at the end of experiment. Results: All treatments resulted in a decrease in glucose and HbA1C compared to pretreatment. No difference in insulin concentration was observed. Serum TC was reduced by (T), while LDL-C decreased with (T) and (T+P) treatments. HDL-C was elevated with all treatments compared to control and (OHA) groups. MDA decreased with all treatments. TOAC was elevated with (T) and (T+P) but not with (P) treatment. Conclusion: Administration of Turmeric, Probiotics, and T+P combination were capable in attenuating hyperglycemia, dyslipidemia and oxidative stress initiated by STZ. However, (T) and (T+P) exerted a more potent hypolipidemic, and antioxidative effect compared to (P) alone.
Diabetes, Oxidative Stress, Probiotics, Curcumin, Supplement
To cite this article
Badkook Maha, Antidiabetic Potential of Turmeric with/without Fermented Milk Enriched with Probiotics in Diabetic Rats, American Journal of Biomedical and Life Sciences. Vol. 1, No. 1, 2013, pp. 1-7. doi: 10.11648/j.ajbls.20130101.11
Folli F, Corradi D, Fanti P, Davalli A, Paez A, Giaccari A, Perego C, Muscogiuri G The role of oxidative stress in the pathogenesis of type 2 diabetes mellitus micro- and macrovascular complications: avenues for a mechanistic-based therapeutic approach. Curr Diabetes Rev. 2011; 7(5): 313-24.
Kempaiah RK, Srinivasan K. Beneficial influence of dietary curcumin, capsaicin and garlic on erythrocyte integrity in high-fat fed rats. J Nutr Biochem. 2006; 17(7) 471– 478.
Joe B, Vijaykumar M, Lokesh BR. Biological properties of curcumin-cellular and molecular mechanisms of action. Crit Rev Food Sci Nutr 2004; 44(2): 97-111.
Pari L, Murugan P. Effect of tetrahydrocurcumin on blood glucose, plasma insulin and hepatic key enzymes in streptozotocin induced diabetic rats. J Basic Clin Physiol Pharmacol 2005; 16: 257–274.
Rungseesantivanon S, Thenchaisri N, Ruangvejvorachai P, Patumraj S. Curcumin supplementation could improve diabetes-induced endothelial dysfunction associated with decreased vascular superoxide production and PKC inhibition. BMC Complement Altern Med. 2010; 10: 57-62.
Babu SP, Srinivasan K. Hypolipidemic action of curcumin, the active principle of turmeric Curcuma longa in streptozotocin induced diabetic rats. Mol and Cellular Biochem. 1997; 166: 169–175.
Srinivasan K, Manjunatha H. Hypolipidemic and antioxidant effects of curcumin and capsaicin in high-fat–fed rats. Can J Physiol Pharmacol 2007; 85:588-96.
Eshrat HM, Hussain A. Hypoglycemic, hypolipidemic and antioxidant properties of combination of Curcumin from CURCUMA LONGA and partially purified product from ABROMA AUGUSTA, in Streptozotocin induced diabetes. Indian J Clin Biochem. 2002; 17 (2): 33-43.
Kempaiah RK, Srinivasan K. Influence of dietary curcumin, capsaicin and garlic on the antioxidant status of red blood cells and the liver in high-fat-fed rats. Ann Nutr Metab. 2004; 48(5): 314-20.
Jang E-M, Choi M-S, Jung UJ, Kim M-J, Kim H-J, Jeon S-M, et al. Beneficial effects of curcumin on hyperlipidemia and insulin resistance in high-fat–fed hamsters. Metab Clin and Exp. 2008; 57: 1576–1583.
Iannitti T, Palmieri B.Therapeutical use of probiotic formulations in clinical practice.Clin Nutr. 2010; 29(6):701-25.
Nagpal R, Yadav H, Puniya AK, Singh K, Jain S, Marotta F. Potential of probiotics and prebiotics for synbiotic functional dairy foods. Int. J Probiotics and Prebiotics 2007; 2:75–84.
Sanders TAB. Food production and food safety. BMJ 1999; 318(7199):1689–1693.
Nagpal R, Behare PV, Kumar M, et al. Milk, milk products and disease free health: an updated overview. Crit Rev Food Sci and Nutr 2011; 52(4):1549–52.
Pereira DIA, Gibson GR. Effects of consumption of probiotics and prebiotics on serum lipid levels in humans. Crit Rev Biochem and Mol Biol 2002; 37(4):259–281.
Cani PD, Delzenne NM. The role of the gut microbiota in energy metabolism and metabolic disease. Curr Pharm Des. 2009; 15(13): 1546-58.
Tonyushkina K, Nichols JH. Glucose meters: a review of technical challenges to obtaining accurate results, J Diabetes Sci Technol. 2009; 3: 971-980.
Nuttall FQ (1998) Comparison of percent total GHb with percent HbA1c in people with and without known diabetes. Diabetes Care 1998; 21(9):1475-80.
SchettlerG, Nussel E. Enzymatic calorimetric determination of high density lipoprotein cholesterol by CHOD-PAP method, Arb. Med Soz. Med Prov Med. 1975;10:25.
Izawa S, Okada M, Matsui H, Horita Y. A new direct method for measuring HDL cholesterol which does not produce any biased values, J. Med. Pharm. Sci 1997; l37:1385-1388.
Fassati P, Prencipe L. Triglyceride enzymatic colorimetric method. Clin Chem 1982; 28: 2077.
Friedewald, W.T., Levy, R.I., Fredrickson, D.S. Estimation of the concentration of low-density lipoprotein cholesterol in plasma without use of preparative ultracentrifuge. Clin Chem 1972; 18 (6): 499– 502.
Ohkawa, H., Ohishi, N., & Yagi, K. Assay for lipid peroxides in animal tissue by thiobarbituric acid reaction. Analytical Biochemistry 1979; 95(2): 351–58.
Koracevic, D; Koracevic, G; Djordjevic, V; Andrejevic, S; Cosic, V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54; 5 : 356-61.
Eastham RD. Biochemical values in clinical medicine. 7th ed. Bristol, England John Wright & Sons Ltd. (1985)
Pari L, Murugan P. Antihyperlipidemic effect of curcumin and tetrahydrocurcumin in experimental type 2 diabetic rats. Ren Fail. 2007; 29(7): 881-9.
Menon VP, Sudheer AR. Antioxidant and anti-inflammatory properties of curcumin. Adv Exp Med Biol. 2007;595:105-25.
Fujiwara H, Hosokawa M, Zhou X, Fujimoto S, Fukuda K, Toyoda K, et al. Curcumin inhibits glucose production in isolated mice Hepatocytes. Diabetes Res and Clin Pract. 2008; 80:185-91.
Shehzad A, Ha T, Subhan F, Lee YS. New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr. 2011; 50(3):151-61.
Al-Salami H, Butt G, Fawcett JP, Tucker IG, Golocorbin-Kon S, Mikov M. Probiotic treatment reduces blood glucose levels and increases systemic absorption of gliclazide in diabetic rats. Eur J Drug Metab Pharmacokinet. 2008; 33(2):101-6.
Soudamini KK, Unnikrishnan MC, Soni KB, Kuttan R. Inhibition of lipid
peroxidation and cholesterol levels in mice by curcumin. Indian J Physiol Pharmacol. 1992; 36:239–43.
Arafa HM. Curcumin attenuates diet-induced hypercholesterolemia in rats. Med Sci Monit. 2005; 11:BR228–34.
Ramirez-Tortosa MC, Mesa MD, Aguilera MC, Quiles JL, Baro L. Oral administration of a turmeric extract inhibits LDL oxidation and has hypocholesterolemic effects in rabbits with experimental atherosclerosis. Atherosclerosis 1999; 147:371–8.
Usman HA. Bile tolerance, taurocholate deconjugation, and binding of cholesterol by Lactobacillus gasseri strains. J Dairy Sci. 1999; 82(2):243–248.
Sadrzadeh-Yeganeh H, Elmadfa I, Djazayery A, Jalali M, Heshmat R, Chamary M. The effects of probiotic and conventional yoghurt on lipid profile in women. Br J Nutr. 2010; 103(12):1778-83.
Meghana K, Sanjeev G, Ramesh B. Curcumin prevents streptozotocin-induced islet damage by scavenging free radicals: A prophylactic and protective role. European J Pharm. 2007; 577: 183–191.
Sundaram, R.K., Bhaskar, A., Vijayalingam, S., Viswanatthan, M., Moha, R., Shanmugasundaram, K.R. Antioxidant status and lipid peroxidation in type II diabetes mellitus with and without complications. Clin Sci. 1996; 90, 255–60.
Murugan P, Pari L. Antioxidant effect of tetrahydrocurcumin in streptozotocin–nicotinamide induced diabetic rats. Life Sciences 2006; 79:1720–1728.
Al-Suhaimi EA, Al-Riziza NA, Al-Essa RA. Physiological and therapeutical roles of ginger and turmeric on endocrine functions. Am J Chin Med. 2011; 39(2):215-31.
Fabian E, Elmadfa I. The effect of daily consumption of probiotic and conventional yoghurt on oxidant and anti-oxidant parameters in plasma of young healthy women. Int J Vitam Nutr Res. 2007; 77(2):79-88.
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