Volume 2, Issue 3, June 2014, Page: 60-64
Antiplasmodial Activity of Aqueous leaf Extract of Cymbopogon citratus against Plasmodium falciparum Infected Rats
Shekins Osheke Okere, Department of Biochemistry, Bingham University, Karu, Nigeria
Janet Olayemi Sangodele, Department of Biochemistry, Bingham University, Karu, Nigeria; Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Eunice Ogunwole, Department of Physiology, Bingham University, Karu, Nigeria
Moses Dele Adams, Department of Biochemistry, Bingham University, Karu, Nigeria
Mercy Omoye Shafe, Department of Physiology, Bingham University, Karu, Nigeria
Received: Jun. 9, 2014;       Accepted: Jun. 20, 2014;       Published: Jul. 10, 2014
DOI: 10.11648/j.ajbls.20140203.12      View  3311      Downloads  249
In Nigeria, malaria is a major public health problem and there are high cost of the effective antimalarial drugs, poor quality drugs and increased emergence of Plasmodial resistance. Thus, there is a need for alternative source of medicine in malaria treatment and prevention. The antimalarial potential of aqueous leaf extracts of Cymbopogon citratus was investigated in this present study. Twenty five Swiss albino rats with average weight of 30.80g were distributed into five groups (A, B, C, D and E) with five mice per group. Group B was infected with 0.2 ml O+ human parasitized blood of Plasmodium falciparum and 0.1ml Chloroquine (Bini Laboratories Pvt Ltd). Group C, D, and E were infected with 0.2 ml O+ human parasitized blood of Plasmodium falciparum treated with 40 mg/kg, 80 mg/kg and 120 mg/kg of Cymbopogon citratus extracts respectively for three days. The mice infected with 0.2 ml O+ human parasitized blood of Plasmodium falciparum were observed for 72 hours for general symptoms of malaria. The mice tail was punctured, blood was examined under light microscope (x10) resolution) and several malaria parasites were found. Significant decrease of parasitemia levels was observed in 120 mg/kg body weight treated group compared to 0.1 ml Chloroquine the positive control. The result showed that Cymbopogon citratus possessed a good antimalarial property and can be use for prophylactic and chemotherapeutic purposes.
Cymbopogon citratus, Chloroquine, Blood, Plasmodium falciparum
To cite this article
Shekins Osheke Okere, Janet Olayemi Sangodele, Eunice Ogunwole, Moses Dele Adams, Mercy Omoye Shafe, Antiplasmodial Activity of Aqueous leaf Extract of Cymbopogon citratus against Plasmodium falciparum Infected Rats, American Journal of Biomedical and Life Sciences. Vol. 2, No. 3, 2014, pp. 60-64. doi: 10.11648/j.ajbls.20140203.12
WHO. Training module on malaria control: Malaria Case Management Guide for Participants. World Health Organization (WHO); 2013. Available: http://apps.who.int/iris/bitstream/10665/78070/1/9789241503976_eng.pdf
KFF. The Global Malaria Epidemic- Fact Sheet: US Global Health Policy. (2013) The Henry J. Kaiser Family Foundation; Available: http://kaiserfamilyfoundation.files.wordpress.com/2012/10/7882-04-2.pdf.
Cheel J, Theoduloz C, Rodríguez J, Schmeda-Hirschmann G (2005). Free radical scavengers and antioxidants from Lemongrass (Cymbopogon citratus (DC.) Stapf.) J. Agric. Food Chem. 53: 2511-2517.
Ernst, E. (2008). "Chiropractic: a critical evaluation". Journal on Pain Symptom Management 35 (5): 544-6213.
Huynh KP, Maridable J, Gaspillo P, Hasika M, Malaluan R, Kawasaki J (2008). Essential oil from lemon grass extracted by supercritical carbon dioxide and steam distillation. The Phillippine Agric. Sci. 91: 36-41.
Pearson O (2010). The Antibacterial Properties of Essential Oils. http://www.livestrong.com/article/168697-the-antibacterialproperties-of-essential-oils
Brian TS, Ikhlas AK (2002). Comparison of extraction methods for marker compounds in the essential oil of lemongrass by GC. J. Agric. Food Chem. 50: 1345-1349.
Aibinu I, Adenipekun T, Adelowotan T, Ogunsanya T, Odugbemi T (2007). Evaluation of the antimicrobial properties of different parts of Citrus aurantifolia (lime fruit) as used locally. Afr. J. Tradit. CAM. 2: 185-190.
Depken KL (2011). Properties of Lemon Grass. http://www.ehow.com/about_5382246_properties-lemongrass. html.
Bonjar, G.H.S & Farrokhi, P.,R. (2004). Antibacillus activity of some plant used intraditional medicine of Iran. Nigerian Journal on National Prod. Med. (8): 34-39.4.
Rios, J.L & Recio, M., C. (2005). Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology 100: 80-84.
Tchoumbougnang, F; Zollo, P.H.A; Dagne, E; Mekonnen, Y., Planta Med., (2005), 71, 20-23.
Fair, J.D & Kormas, C.M. (2008). Journal on Chromatography, 1211 (1-2), 49-54.
Onawunmi G.O. (1989) Evaluation of the antimicrobial activity of citral. Lett Appl Microbiol 9:105-8.24.
Wannissorn B., Jarikasem S., Soontorntanasart T. (1996) Antifungal activity of lemon grass and lemon grass oil cream. Phytotherapy Res 10:551-4.40.
Schuck V.J.A., Fratini M., Rauber C.S., (2001) Avaliação da atividade antimicrobiana de Cymbopogon citratus Revista Brasileira de Ciências Farmacêuticas 37:45-9
Paranagama P.A., Abeysekera K.H.T., Abeywickrama K., Nugaliyadde L. (2003) Fungicidal and anti-aflatoxigenic effects of the essential oil of Cymbopogon citratus (DC.) Stapf (lemon grass) against Aspergillus flavus Link. Isolated from stores rice. Lett Appl Microbiol 37:86-90
Wannissorn B., Jarikasem S., Siriwangechain,T., Thubthimthed, S. (2005) Antibacterial properties of essential oils from Thain medicinal plants. Fitoterapia 76:233-6.
Minami M., Kita M., Nakaya T., (2003) The inhibitory effect of essential oils on Herpes simples virus type-1 replication in vitro Microbiol Immunol; 47: 681-4.
Coles GC. (1997) Nematode control practices and anthelminthic resistance on British sheep farms. Vet. Rec; 141: 91–93.8.
Deore S. L., S. S. Khadabadi, Kamdi K. S., Ingle V. P., Kawalkar N. G. Sawarkar P. S, Patil U. A, Vyas A. (2009) In vitro Anthelmintic activity of Cassia tora. International Journal of Chem Tech Research. Vol.1, No.2, pp 177-179,
Nakamura, Y.; Miyamoto. M.; Murakami, A.; Ohigashi, H.; Osawa, T & Uchida. K. (2003). A phase 11 detoxification enzyme inducer from lemon grass: identification of citral. 23.
Onawunmia, G.O; Yisak, W. A. and Ogunlana, E.O., Journal of Ethnopharmacology, (1984), 12, 279-286.
Bate Smith E.C. (1962) The phenolic constituent of plants and their taxonomic significance, dicotyledons. J Linn Soc Bot 58:95‐103.3.
Melo, S.F; Soares, S.F; da Costa, R.F; da Silva, C.R; de Oliveira, M.B; Bezerra R.J; Caldeira-de-Araujo A; and Bernardo-Filho M., (2001) Mutat Res, 496, 33-38.
Syed, M; Khalid, M.R. and Chaudhary, F.M., (1990) Pak J Sci Ind Res., 33, 529-531.
Browse journals by subject