Volume 5, Issue 5, October 2017, Page: 88-91
Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E
Alceu Afonso Jordao, Division of Nutrition, Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Thais Helena Monteiro, Division of Nutrition, Department of Internal Medicine, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
Raquel Alves dos Santos, Nucleus of Research in Sciences and Technology, University of Franca, Franca, Brazil
Guilherme Vannucchi Portari, Department of Nutrition, Federal University of Triangulo Mineiro, Uberaba, Brazil
Received: Apr. 11, 2017;       Accepted: May 23, 2017;       Published: Oct. 18, 2017
DOI: 10.11648/j.ajbls.20170505.11      View  1991      Downloads  191
The metabolism of ethanol is directly related to oxidative stress, and its ingestion leads to the formation of reactive oxygen species (ROS) such as hydroxyl radicals, superoxide and hydrogen peroxide. Vitamin E has been widely used as an antioxidant; when administered in large doses it is deposited in the liver and then excreted in the bile, urine and feces. The objective of the present study was to evaluate the rate of excretion of fecal vitamin E in relation to its concentrations in serum and liver, and its role as a protective antioxidant against DNA damage induced by acute ethanol consumption. Wistar rats were divided into four groups receiving food and water ad libitum for 4 days plus the following treatments: Control (CG, n = 10 ) no treatment; Ethanol (ET, n = 10 ), receiving an acute ethanol dose intraperitoneally in the amount of 5 g/kg; vitamin E (VE, n = 10) receiving a high oral dose of vitamin E within the first three days in the amount of 100 tocopherol mg/kg body weight; ethanol plus vitamin E (VE + ET, n = 10 ) receiving both the ethanol and vitamin E doses. Higher concentrations of vitamin E were observed in the blood and liver of the animals in the groups that received vitamin E supplementation, independent of the presence or absence of ethanol. Concomitantly, these groups were also those with the highest concentration of the vitamin in the stool. The rate of DNA damage was higher in the groups that received ethanol with or without supplemental vitamin E. However, the rate of damage was lower in the group that received vitamin E supplementation than in the group that did not. The present results show that vitamin E has a protective effect against DNA damage induced by ethanol by reducing the extent of DNA damage.
Vitamin E, Ethanol, Antioxidants, DNA Damage, Supplementation
To cite this article
Alceu Afonso Jordao, Thais Helena Monteiro, Raquel Alves dos Santos, Guilherme Vannucchi Portari, Oxidative Damage in Rats Receiving Ethanol and Supplemented with Vitamin E, American Journal of Biomedical and Life Sciences. Vol. 5, No. 5, 2017, pp. 88-91. doi: 10.11648/j.ajbls.20170505.11
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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