Volume 6, Issue 4, August 2018, Page: 78-84
Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae)
Ablassé Rouamba, Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Moussa Compaoré, Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Maurice Ouédraogo, Laboratory of Animal Physiology, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Martin Kiendrebeogo, Laboratory of Applied Biochemistry and Chemistry, UFR-SVT, University Ouaga I Pr Joseph KY-ZERBO, Ouagadougou, Burkina Faso
Received: Aug. 3, 2018;       Accepted: Aug. 31, 2018;       Published: Oct. 6, 2018
DOI: 10.11648/j.ajbls.20180604.13      View  259      Downloads  25
Abstract
DNA is continuously degraded by numerous genotoxic agents including intracellular reactive oxygen species produced by cell metabolism and exogenous environmental pollutants. These genotoxic agents destroy the DNA integrity leading to carcinogenesis or cell death. An increased consumption of vegetables, fruits and other foods rich in antioxidant compounds can protect DNA from oxidative damage and prevent cell carcinogenesis. This study was designed to investigate in vitro the genoprotective and DNA repair activities of the fruit pulp ethanol extract from Detarium microcarpum against two known mutagenic agents such as hydrogen peroxide and methyl methane sulfonate. To assess the genoprotective and DNA repair activities of extract, human lymphocytes in culture were treated with the extract before or after the genotoxic agent exposing. The amount of DNA damages was assessed by using the standard comet assay. The fruit pulp extract in concentration up to 500 µg/mL, compared to vehicle didn’t affect the integrity of DNA. Interestingly, the genotoxic effects of hydrogen peroxide and methyl methane sulfonate on human lymphocytes were significantly reduced by the extract pre-treatment. In addition, the DNA damages induced by hydrogen peroxide and methyl methane sulfonate were repaired further to the extract addition. The fruit pulp ethanol extract from Detarium microcarpum contains bioactive compounds that can preserve the integrity of DNA from the deleterious effects of genotoxic agents. The daily intake of this fruit pulp as food supplement could prevent DNA damages and carcinogenesis.
Keywords
Detarium microcarpum, Genoprotection, DNA Repair, Hydrogen Peroxide, Methyl Methane Sulfonate
To cite this article
Ablassé Rouamba, Moussa Compaoré, Maurice Ouédraogo, Martin Kiendrebeogo, Genoprotective and DNA Repair Activities of Fruit Pulp Ethanol Extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae), American Journal of Biomedical and Life Sciences. Vol. 6, No. 4, 2018, pp. 78-84. doi: 10.11648/j.ajbls.20180604.13
Copyright
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Reference
[1]
M. Gafrikova, E. Galova, A. Sevcovicova, P. Imreova, P. Mucaji, and E. Miadokova, “Extract from armoracia rusticana and its flavonoid components protect human lymphocytes against oxidative damage induced by hydrogen peroxide,” Molecules, vol. 19, no. 3, pp. 3160–3172, 2014.
[2]
P. Chao, W. Huang, S. Hu, H. Lo, K. Lin, and M. Huang, “Indigenous Purple Vegetable Extracts Protect against Hydrogen Peroxide-Induced DNA Damage in Human Lymphocytes,” Food Nutr. Sci., vol. 2013, no. August, pp. 62–70, 2013.
[3]
A. A. Boligon, M. R. Sagrillo, L. F. Machado, O. D. S. Filho, M. M. Machado, I. B. M. Da Cruz, and M. L. Athayde, “Protective effects of extracts and flavonoids isolated from scutia buxifolia reissek against chromosome damage in human lymphocytes exposed to hydrogen peroxide,” Molecules, vol. 17, no. 5, pp. 5757–5769, 2012.
[4]
P. V. Mahadimane and V. Vasudev, “Effect of methyl methane sulfonate on Ehrlich ascites carcinoma cells : dose effect relationships,” Int. J. Life Sci. Pharma Res., vol. 3, no. 2, pp. 22–31, 2013.
[5]
K. Nisha and R. K. Deshwal, “Antioxidants and their protective action against DNA damage,” Int. J. Pharm. Pharm. Sci., vol. 3, no. SUPPL. 4, pp. 28–32, 2011.
[6]
G. Pace, P. Lima, F. Vianello, C. R. Corrêa, R. Arnoux, D. S. Campos, and M. G. Borguini, “Polyphenols in Fruits and Vegetables and Its Effect on Human Health,” Food Nutr. Sci., vol. 5, no. 5, pp. 1065–1082, 2014.
[7]
L. Wang, S. Gao, W. Jiang, C. Luo, M. Xu, L. Bohlin, M. Rosendahl, and W. Huang, “Antioxidative dietary compounds modulate gene expression associated with apoptosis, DNA repair, inhibition of cell proliferation and migration,” Int. J. Mol. Sci., vol. 15, no. 9, pp. 16226–16245, 2014.
[8]
F. Bamisaye, E. Ajani, I. Nurain, K. Adebisi, R. Quadri, and J. Minari, “Evaluation of Growth Performance of Rats Fed With Sweet Detar, Detarium Microcarpum Fruit as Supplementary Feed Ingredient,” J. Environ. Sci. Toxicol. Food Technol., vol. 8, no. 11, pp. 115–121, 2014.
[9]
C. O. Obun, S. M. Yahaya, A. A. Kibon, O. A. Olafadehan, and S. D. Alison, “Evaluation of Detarium microcarpum pulp meal as feed ingredient in rabbits diets,” Electron. J. Environ. Agric. Food Chem., vol. 9, no. 2, pp. 308–314, 2010.
[10]
A. L. Cavin, A. E. Hay, A. Marston, H. Stoeckli-Evans, R. Scopelliti, D. Diallo, and K. Hostettmann, “Bioactive diterpenes from the fruits of Detarium microcarpum,” J. Nat. Prod., vol. 69, no. 5, pp. 768–773, 2006.
[11]
F. Kini, S. Ouédraogo, and I. Pierre, “Propriétés nutritionnelles et thérapeutiques du fruit de Detarium microcarpum Guill . et Perr,” Mol. Med., vol. 4, no. 1, pp. 26–30, 2010.
[12]
A. Rouamba, M. Compaore, M. Ouédraogo and M. Kiendrebeogo.'' Human lymphocyte-protective effects of an ethanol extract from Detarium microcarpum Guill. and Perr. (Caesalpiniaceae) fruit pulp. Antioxidants vol. 104, pp. 1-7.
[13]
A. Velalopoulou, S. Tyagi, R. a. Pietrofesa, E. Arguiri, and M. Christofidou-Solomidou, “The flaxseed-derived lignan phenolic secoisolariciresinol diglucoside (SDG) protects non-malignant lung cells from radiation damage,” Int. J. Mol. Sci., vol. 17, no. 1, pp. 1–15, 2015.
[14]
T. S. Kumaravel and A. N. Jha, “Reliable Comet assay measurements for detecting DNA damage induced by ionising radiation and chemicals,” Mutat. Res. - Genet. Toxicol. Environ. Mutagen., vol. 605, no. 1–2, pp. 7–16, 2006.
[15]
G. Kaur, “Evaluation of DNA Damage by Methyl Methane Sulfonate in Allium cepa Root Cells by Comet Assay,” World J. Environ. Biosci., vol. 3, no. 2, pp. 109–115, 2014.
[16]
N. K. Bhagyanathan and J. E. Thoppil, “Genotoxic potential of Cynanchum sarcomedium meve & liede coupled with its modulatory action on oxidative-stress mediated genotoxicity by hydrogen peroxide,” Turkish J. Biol., vol. 40, no. 1, pp. 120–129, 2016.
[17]
A. Lamien-Meda, E. C. Lamien, M. Y. M. Compaoré, N. T. R. Meda, M. Kiendrebeogo, B. Zeba, F. J. Millogo, and G. O. Nacoulma, “Polyphenol content and antioxidant activity of fourteen wild edible fruits from Burkina Faso,” Molecules, vol. 13, pp. 581–594, 2008.
[18]
M. B. Prava, M. M. Ranjan, and T. N. Kanti, “Antimutagenic Effects of Ascorbic Acid against the Genotoxicity of Dimethyl Sulphate in Drosophila,” Univers. J. o Res. Environ. Res. Technol., vol. 2, no. 1, pp. 77–84, 2012.
[19]
S. J. Hosseinimehr, A. Mahmoudzadeh, and A. Rafiei, “Arginine increases genotoxicity induced by methyl methanesulfonate in human lymphocytes,” Cytotechnology, vol. 65, no. 3, pp. 379–384, 2013.
[20]
H. Kizilet, C. Kasimoǧlu, and H. Uysal, “Can the Rosa canina plant be used against alkylating agents as a radical scavenger?,” Polish J. Environ. Stud., vol. 22, no. 4, pp. 1263–1267, 2013.
[21]
P. F. De Oliveira, L. F. Leandro, G. Montanheiro, J. K. Bastos, A. A. D. S. Filho, and D. C. Tavares, “Baccharin Prevents Genotoxic Effects Induced by Methyl Methanesulfonate and Hydrogen Peroxide in V79 Cells,” J. Food Sci., vol. 77, no. 8, 2012.
[22]
M. C. Lazzé, R. Pizzala, M. Savio, L. a. Stivala, E. Prosperi, and L. Bianchi, “Anthocyanins protect against DNA damage induced by tert-butyl-hydroperoxide in rat smooth muscle and hepatoma cells,” Mutat. Res. - Genet. Toxicol. Environ. Mutagen., vol. 535, no. 1, pp. 103–115, 2003.
[23]
P. Fortini, B. Pascucci, F. Belisario, and E. Dogliotti, “DNA polymerase β is required for efficient DNA strand break repair induced by methyl methanesulfonate but not by hydrogen peroxide,” Nucleic Acids Res., vol. 28, no. 16, pp. 3040–6, 2000.
Browse journals by subject