Volume 6, Issue 5, October 2018, Page: 96-102
Biosynthesis, Characterization and Application of Chitin Nanoparticle with Cassia auriculata
Bhuvaneswari Dhandapani, PG and Research Department of Biotechnology, Women`s Christian College, Chennai, India
Priya Rajagopal Iyer, PG and Research Department of Biotechnology, Women`s Christian College, Chennai, India
Received: Aug. 1, 2018;       Accepted: Oct. 5, 2018;       Published: Nov. 9, 2018
DOI: 10.11648/j.ajbls.20180605.11      View  995      Downloads  153
Prawns are a common food for humans all around the World. Particularly the prawn exists naturally in south East Asia. Prawn shells contain ash, fibre, minerals and proteins. Prawns like a Shrimps. It’s a Dendrobranchiata family. Prawn shells were collected from Chindaripet market. Deproteinization followed by demineratisation was carried out to obtain the final product chitin. Cassia auriculata is a legume tree in the subfamily Caesalpinioideae. It contains antioxidants, cardiac Glycosides, anthraguinones, tannins, carbohydrates, alkaloids, phenols, saponin and flavonoids. Cassia auriculata flower has anti- bacterial, anti- diabetic property, the flower taste is stringent, pungent and bitter. Cassia auriculata flowers were dried under room temperature for 2 to 3weeks flowers and grounded with mechanical blender. Then chitin and silver nanoparticles were prepared along with Cassia auriculata flower extract which was extracted with both ethanol and water. Scanning Electron Microscope (SEM) was done by chitin nanoparticles to identify the particle size and morphology. Fourier Transform Infrared Spectroscopy (FTIR) was done to identify the active compounds. Chitin nanoparticles were used widely in various applications that evaluates anti-microbial activity using E. coli, Staphylococcusaureus, Salmonella typhi, Klebsiella pneumoniae, Staphylococcus mutans, Entero coccus spp, then anti-inflammatory activity gives better inhibition, anti-larvae activity using mosquito larvae. Cassia auriculata (fresh, ethanol) nanoparticles killed all larvae, anti-coagulant and thrombolytic activity were observed.
Prawn Shell, Cassia Auriculata, Chitin, Nano Particles, SEM, FTIR
To cite this article
Bhuvaneswari Dhandapani, Priya Rajagopal Iyer, Biosynthesis, Characterization and Application of Chitin Nanoparticle with Cassia auriculata, American Journal of Biomedical and Life Sciences. Vol. 6, No. 5, 2018, pp. 96-102. doi: 10.11648/j.ajbls.20180605.11
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abdou E. Set al. Extraction and characterization of chitin and chitosan from local sources, Bioresources Technology, 2008, 99.
C. Anushia et al., Antibacterial and Antioxidant Activities in Cassia auriculata, Global Journal of Pharmacology, 3 (3): 127-130, 2009.
AmruteshS. et al., Cassia auriculata: Aspects of Safety Pharmacology and Drug Interaction, December 2009, 8 pages.
Devadoa Kumarasamy Raja et al., In vitro antimicrobial activity and phytochemical analysis of Cassia auriculata Linn, 2013.
Guruprasad C. et al., A Phytopharmacological Review of plant – Cassia auriculata, International Journal of Pharmaceutical & Biological Archives 2015; 6 (6): PP1- 9.
Kalut S. A. et al., Enhancement of degree of deacetylation of chitin in chitosan production, B. Chemical Engineering, University Malaysia Pahang, 2008.
M. Monisha, et al., Extraction of Bio Active Compounds from Cassia Auriculata Pods and Leaves and its Medicinal Uses, ISSN: Volume 6 Number 8 (2017) pp 425-434.
M. Kanthimathi et al., Phytochemical screening and Invitro antibacterial potential of Cassia auriculata Linn. Flowers Against Pathogenic Bacteria. Sri paramakalyanicentre for environmental sciences, Manonmaniyamsundaranar university, Alwarkurichi . IRJPBS October 2014; 1(1): 45-56.
Mohammad Shahriar et al., Phytochemical screenings, thrombolytic activity and antimicrobial properties of the bark extracts of Averrhoa bilimbi, Journal of Applied Pharmaceutical Science Vol. 3 (03), pp. 094-096, March, 2013.
N. Muruganantham et al., Anti-oxidant and Anti-inflammatory Activity of Cassia auriculata (Flowers), BJPR, 8(1): 1-9, 2015.
Nessa F. et al. A process for the preparation of chitin and chitosan from prawn shell waste, Bangladesh J. Sci. Ind. Res., 2010.
S. Maneemegalai et al., Evaluation of Antibacterial Activity of Flower Extracts of Cassia auriculata L. Ethnobotanical Leaflets 14: 182- 92, 2010.
R. Ashokkumar et al., Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian Medicinal plants, Int. J. Curr. Microbiol. App. Sci (2014) 3(1): 395-406.
Sachin Chaudhary et al., Phytochemical Analysis and Assessment of In-vitro Anthelmintic Activity of Cassia auriculata Linn leaves, AJPCT [2], 2014, 161-167.
Senthil Rani SoundharaRajan et al., Chemical composition and characterization studies of Cassia auriculata flower extract, Int Pharm PharmSci, Vol 6, Issue 4, 327-330.
V. Kavimani et al., Antibacterial activity of Cassia auriculata Linn, J. Chem. Pharm. Res., 2015, 7(9):479-485
Vinh Quang Nguyen, Preparation of Size-Controlled Silver Nanoparticles and
Chitin-Based Composites and Their Antimicrobial Activities, Hindawi Publishing Corporation
Volume 2013, Article ID 693486, 7 pages, 2013
Browse journals by subject