Volume 7, Issue 6, December 2019, Page: 143-147
Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis
Xi Qinhua, Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Teng Yajie, Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Li Yueqin, Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Dai Juan, Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China
Zhang Guangbo, Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, China
Chen Weichang, Department of Gastroenterology, First Affiliated Hospital of Soochow University, Suzhou, China; Institute of Clinical Immunology, First Affiliated Hospital of Soochow University, Suzhou, China
Received: Sep. 14, 2019;       Accepted: Oct. 29, 2019;       Published: Nov. 13, 2019
DOI: 10.11648/j.ajbls.20190706.13      View  413      Downloads  114
Abstract
This study was performed to study the effects of astragalus polysaccharides (APS) in the treatment of ulcerative colitis (UC), and to explore whether myeloid-derived suppressor cells (MDSC) involve in this process. UC model was established by dextran sulfate sodium salt (DSS) inducement in mouse. Then the effects of APS on UC was evaluate at the levels of cytology and molecular biology: exploring the influence of APS on MDSC by analyzing the levels of MDSC before and after the treatments of APS; evaluating the therapeutic effects of APS on UC by pathologic histology. Data showed that the levels of MDSC in bone marrow, spleen, peripheral blood of UC mice were significantly decreased after intervention with APS, indicating APS inhibited the level of MDSC significantly. After depletion bone marrow cells in mice, we further found that the therapeutic effects of APS in MDSC- group was significantly reduced compared with MDSC+ group. It was discovered through pathological analysis that, compared with UC model group, APS intervention group mainly manifested as reduced infiltrating acute and chronic inflammatory cells, necrotic epithelial cells and epithelial ulceration. Therefore, APS could reduce the inflammatory cell infiltration in colonic tissues of UC model mice, repair the damaged colonic mucosa, and promote ulcer healing. In conclusion, APS has a potential application in the treatment of ulcerative colitis, and is dependent or partially dependent on MDSC to achieve this effects.
Keywords
Astragalus Polysaccharides, Ulcerative Colitis, Mouse Model, MDSC
To cite this article
Xi Qinhua, Teng Yajie, Li Yueqin, Dai Juan, Zhang Guangbo, Chen Weichang, Empirical Study on the Therapeutic Effect of Astragalus Polysaccharides in a Mouse Model of Ulcerative Colitis, American Journal of Biomedical and Life Sciences. Special Issue: Inflammatory Bowel Disease. Vol. 7, No. 6, 2019, pp. 143-147. doi: 10.11648/j.ajbls.20190706.13
Copyright
Copyright © 2019 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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