Volume 8, Issue 5, October 2020, Page: 126-130
Development of a Thin Layer Chromatography Method for Simultaneous Detection of Chloroquine, Azithromycin and Paracetamol in Counterfeit Tablets Used Against Covid-19
Faida Losa, Faculty of Medicine and Pharmaceutical Sciences, University of Kisangani, Kisangani, Democratic Republic of Congo
Timothy Mavanga Mabaya, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Jocelyn Mankulu Kakumba, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Michael Tambwe Ngoyi, National Laboratory for Quality Control of Medicines, Public Health Ministry, Kinshasa, Democratic Republic of Congo
Floribert Balazirhe, National Laboratory for Quality Control of Medicines, Public Health Ministry, Kinshasa, Democratic Republic of Congo
Margaret Borive, Faculty of Medicine and Pharmaceutical Sciences, University of Kisangani, Kisangani, Democratic Republic of Congo
Didi Mana Kialengila, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Jeremiah Mbinze Kindenge, Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of Congo
Received: Jul. 9, 2020;       Accepted: Aug. 11, 2020;       Published: Aug. 31, 2020
DOI: 10.11648/j.ajbls.20200805.11      View  44      Downloads  31
Abstract
Corona Virus Disease 2019 (Covid-19) is an infectious disease manifested by fever, rate and difficulty in breathing that can lead to death. This pandemic has just paralyzed the world, causing more deaths amongst vulnerable people such as the elderly and people with immune problems. Several therapeutic agents are being tested for the treatment of Covid-19. The combination of Chloroquine, Azithromycin and Paracetamol is identified as a promising therapeutic candidate for Covid-19. To ensure the success of this therapy, medicines of high quality play a key role. Unfortunately, we are not totally spared from trafficking of poor quality pharmaceutical products. As a contribution to try solving this challenge, a convenient, rapid and simple Thin Layer Chromatography method (TLC), which permits the simultaneous determination of Chloroquine, Azithromycin and paracetamol has been developed. The Chromatographic separation was achieved on 60 F silica gel plate using a mixture of methanol-25% ammonia (100:1.5, v/v). Iodine vapor and ultraviolet light at 254 nm were used for the visualization of the spots. The developed method was successfully applied to determine Chloroquine in 23 samples supposed to contain Chloroquine marketed in DRC. The results showed that 50% of the samples analyzed were non-compliant and should probably contain paracetamol instead of Chloroquine. It suggested that the optimized method could be used for routine quality assessment of Chloroquine, Azithromycin or Paracetamol in pharmaceutical preparations. Since counterfeiting of medicines continues to be a real problem in developing countries as demonstrated by this result, the development of simple and convenient analytical method that allowed the rapid analysis of medicines used in Covid-19 therapy is more than urgent.
Keywords
Thin Layer Chromatography, Simultaneous Detection, Counterfeit Tablets, Covid-19
To cite this article
Faida Losa, Timothy Mavanga Mabaya, Jocelyn Mankulu Kakumba, Michael Tambwe Ngoyi, Floribert Balazirhe, Margaret Borive, Didi Mana Kialengila, Jeremiah Mbinze Kindenge, Development of a Thin Layer Chromatography Method for Simultaneous Detection of Chloroquine, Azithromycin and Paracetamol in Counterfeit Tablets Used Against Covid-19, American Journal of Biomedical and Life Sciences. Vol. 8, No. 5, 2020, pp. 126-130. doi: 10.11648/j.ajbls.20200805.11
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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