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Age-Dependent Change in Aldo-Keto Reductases Composition in the Blood of Rats
Vadim Vyacheslavovich Davydov,
Anatoliy Ivanovich Bozhkov,
Evgeniya Romanovna Grabovetskaya
Issue:
Volume 2, Issue 6-1, December 2014
Pages:
1-4
Received:
3 August 2014
Accepted:
6 August 2014
Published:
13 August 2014
Abstract: Resistance of the organism to the stress injury changes during ontogenesis. In this regard, the incidence of cardiovascular, central nervous, endocrine systems, etc. increases at certain stages of individual development. Taking into account the fact that the development of stress-caused lesions is associated with accumulation of carbonyl products of free radical oxidation in cells, we have suggested that the one of the reasons for this phenomenon is age-related changes in the efficiency of the scavenging of endogenous aldehydes in the organism. In view of this fact, spectrum of aldo-keto reductases in blood serum of rats at different stages of ontogenesis was investigated by means of electrophoresis. Identical changes in the composition of aldo-keto reductases spectrum of blood in early immature age and in aging have been shown. Change in aldo-keto reductases spectrum modulates the role of reductive pathway of endogenous aldehydes scavenging in the organism. Thus, in child age and aging efficiency of scavenging of carbonyl products of free radical oxidation in cells is limited and especially when they are intensively produced. This results in increase of the susceptibility of the organism to oxidative stress.
Abstract: Resistance of the organism to the stress injury changes during ontogenesis. In this regard, the incidence of cardiovascular, central nervous, endocrine systems, etc. increases at certain stages of individual development. Taking into account the fact that the development of stress-caused lesions is associated with accumulation of carbonyl products o...
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Stem Cells Take Part in Regulation of Prooxidant Activity and Immunity at Liver Fibrosis
Anatoliy Ivanovich Bozhkov,
Elena Mikhajlovna Klimova,
Yuriy Viktorovich Nikitchenko,
Vadim Vyacheslavovich Davydov,
Oxana Vladimirovna Zvyagintseva,
Natalia Igorevna Kurguzova,
Vadim Ivanovich Sidorov,
Alexandr Vladimirovich Naglov
Issue:
Volume 2, Issue 6-1, December 2014
Pages:
5-12
Received:
13 October 2014
Accepted:
20 November 2014
Published:
26 November 2014
Abstract: It has been explored the possibility of correction of prooxidant-antioxidant parameters at rats with experimental liver fibrosis and immunological status of these animals using xenogeneic embryonic stem cells. It is shown that the threefold sequential administration of copper sulfate in dose 1 mg / 100 g of body weight, which amounted to 30% of the lethal dose in 48 hour intervals between administrations induced liver fibrosis in 24 hours after the last administration to 3 month animals. It was found that the development of fibrosis, was accompanied 50 per cent increase in TBA-active products in the mitochondria and 25% of the increase in the microsomes of liver cells. Content of Ig A, IgM, circulating immune complexes (CIC) and the average molecular weight peptides was increased to 2-3 times in the same time. Introduction of xenogenic embryonic stem cells in animals with experimental fibrosis accompanied by decrease in the amount of TBA-active products to almost intact control. It coincided with the 60% increase in the activity of glutathione peroxidase in the mitochondria. Introduction of stem cells to experimental animals provide a normalization of the content of Ig A, Ig M, the CiC and the average molecular weight of the peptides.
Abstract: It has been explored the possibility of correction of prooxidant-antioxidant parameters at rats with experimental liver fibrosis and immunological status of these animals using xenogeneic embryonic stem cells. It is shown that the threefold sequential administration of copper sulfate in dose 1 mg / 100 g of body weight, which amounted to 30% of the...
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Morphological and Functional Positive Effect with the Association of Hyperbaric Oxygen Therapy and Corticoid Against Acute Acoustic Trauma
Gleice Cristina Colombari,
Adriana de Andrade Batista Murashima,
Omar Feres,
Miguel Angelo Hyppolito
Issue:
Volume 2, Issue 6-1, December 2014
Pages:
13-18
Received:
29 October 2014
Accepted:
19 November 2014
Published:
29 November 2014
Abstract: Studies describe the use of corticosteroids as the best alternative to treat acoustic trauma, but recent studies point to their combination with hyperbaric oxygen therapy (HBOT) as the major benefit in lesions by noise. This study aimed to evaluate the association between HBOT and corticoid therapy (CT). Female guinea pigs were exposed to a white noise on 4 kHz at 110 dB SPL during 72 hours and divided into three treatment groups: HBOT, CT and the combination of both therapies (HBOCT). The treatment lasted five days, being a therapy session per day. All animals were exposed to Distortion Product Otoacoustic Emissions and Brainstem Auditory Evoked Potentials in three moments: before and after exposure to the noise and after the treatment. All cochleae were examined by scanning electron microscopy. Although not statistically significant, the anatomical and functional findings concluded that the association HBOCT played a better otoprotective and therapeutic effect compared to those same therapies alone.
Abstract: Studies describe the use of corticosteroids as the best alternative to treat acoustic trauma, but recent studies point to their combination with hyperbaric oxygen therapy (HBOT) as the major benefit in lesions by noise. This study aimed to evaluate the association between HBOT and corticoid therapy (CT). Female guinea pigs were exposed to a white n...
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Differences in the System of Nitrogen Synthesis and Content of Metabolites in Antioxidant Systems of Sportsmen of Different Sex
Nadezhda V. Bogdanovskaya,
Anastasia V. Golubenko
Issue:
Volume 2, Issue 6-1, December 2014
Pages:
19-24
Received:
28 October 2014
Accepted:
29 November 2014
Published:
27 December 2014
Abstract: The research has been conducted on 26 high qualification sportsmen of different sex, adapted to perform protracted physical activity of high intensity. Determined is the presence of gender differences in oxidizing and no oxidizing metabolism of L-arginine, including also the system of nitrogen oxide synthesis (oxidizing de novo and no oxidizing reutilization ways of NO synthesis), on different stages of their contention activity. On the stage of maximal functional possibilities and adaptation (beginning of contention period) for girls no oxidizing metabolism of arginine predominated with participation of arginase above oxidizing degradation of L-arginine with participation of NO-synthase. During the decline of functional possibilities (desadaptations in the middle and at the end of the competitions) the necessary level of nitrogen oxide synthesis is provided for girls mainly due to nitrogen oxide resynthesis from its stable metabolites, while for youths – due to the increase of oxidizing de novo nitrogen oxide synthesis ( as constitutive, also inducible).
Abstract: The research has been conducted on 26 high qualification sportsmen of different sex, adapted to perform protracted physical activity of high intensity. Determined is the presence of gender differences in oxidizing and no oxidizing metabolism of L-arginine, including also the system of nitrogen oxide synthesis (oxidizing de novo and no oxidizing reu...
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Interconnection of Antitoxic and Antioxidant Systems of the Organism under the Action of Natural Low Molecular Complex – Fungidol
Natalia Igorevna Kurguzova,
Anatoliy Ivanovich Bozhkov,
Yuriy Viktorovich Nikitchenko,
Mohammad Ali Yousef Al Begai,
Anatoliy Vladimirovich Goltvyansky,
Mohammad Morshed Ayed Alsardia,
Andrew Anatolievich Bozhkov
Issue:
Volume 2, Issue 6-1, December 2014
Pages:
25-32
Received:
23 December 2014
Accepted:
10 January 2015
Published:
23 January 2015
Abstract: The influence of the natural complex of low molecular weight compounds (fungidol) on antitoxic and antioxidant systems in animals was investigated. It was demonstrated that the introduction to the experimental animals of fungidol in doses a 0.05 ml per 100 g of body weight increases antioxidant enzymes activity in serum and liver, decreases the content of lipids hydroperoxides and other products of free radical reactions. The components of fungidol antioxidant activity correlated with the stability of membranes of erythrocytes in these animals. In the experimental model the toxic effect of copper sulfate was investigated. The increase in the activity of prooxidant system induced by fungidol correlated with its antitoxic effect.
Abstract: The influence of the natural complex of low molecular weight compounds (fungidol) on antitoxic and antioxidant systems in animals was investigated. It was demonstrated that the introduction to the experimental animals of fungidol in doses a 0.05 ml per 100 g of body weight increases antioxidant enzymes activity in serum and liver, decreases the con...
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